I lost my save file

[Iron]

Save isn't in Backup folder, if that's what you mean. It's in C:\Users\Iron\Saved Games\Pokemon Uranium, which the Backups folder is a subfolder of.

Other than that, it would only be in some other location if you moved it yourself. You could try searching uranium.rxdata on your computer.

But you should definitely check your backups too if you haven't already. I don't see how it could possibly have been deleted for real.

Edit: Oh yeah, and check your recycling bin.